Table 2.
Oligonucleotides used for cloning in this study.
| Primer name | Primer sequence |
|---|---|
| E45A fwd | 5'-CAGCATTATCAGCTGGAGCCACCCC-3' |
| E45A rev | 5'-GGGGTGGCTCCAGCTGATAATGCTG-3' |
| T54A fwd | 5'-CCACAAGATTTAAACGCCATGCTAAACACAGTGG-3' |
| T54A rev | 5'-CCACTGTGTTTAGCATGGCGTTTAAATCTTGTGG-3' |
| T54A/N57A fwd | 5'- CCACAAGATTTAAACGCCATGCTAGCCACAGTGGGGGGAC-3' |
| T54A/N57A rev | 5'-GTCCCCCCACTGTGGCTAGCATGGCGTTTAAATCTTGTGG-3' |
| N57A fwd | 5'-CACCATGCTAGCCACAGTGGGGGGAC-3' |
| N57A rev | 5'-GTCCCCCCACTGTGGCTAGCATGGTG-3' |
| Q63A/Q67A fwd | 5'-GTGGGGGGACATGCAGCAGCCATGGCAATGTTAAAAGAGAC-3' |
| Q63A/Q67A rev | 5'-GTCTCTTTTAACATTGCCATGGCTGCTGCATGTCCCCCCAC-3' |
| K70R fwd | 5'-GCAGCCATGCAAATGTTAAGAGAGACCATCAATGAGGAAG-3' |
| K70R rev | 5'-CTTCCTCATTGATGGTCTCTCTTAACATTTGCATGGCTGC-3' |
| N74D fwd | 5'-GAGACCATCGATGAGGAAGCTGCAGAATGG-3' |
| N74D rev | 5'-CCATTCTGCAGCTTCCTCATCGATGGTCTC-3' |
| G89V fwd | 5'-CCAGTGCATGCAGTGCCTATTGCACCAGGCCAG-3' |
| G89V rev | 5'-CTGGCCTGGTGCAATAGGCACTGCATGCACTGG-3' |
| G89V/A92E fwd | 5'-CCAGTGCATGCAGTGCCAATTGAGCCAGGCCAGATGAG-3' |
| G89V/A92E rev | 5'-CTCATCTGGCCTGGCTCAATTGGCACTGCATGCACTGG-3' |
| P90A fwd | 5'-CCAGTGCATGCAGGTGCCATTGCACCAGGCCAGATG-3' |
| P90A rev | 5'-CATCTGGCCTGGTGCAATGGCACCTGCATGCACTGG-3' |
| P90A/A92E fwd | 5'-CCAGTGCATGCAGGCGCAATTGAGCCAGGCCAGATG-3' |
| P90A/A92E rev | 5'-CATCTGGCCTGGCTCAATTGCGCCTGCATGCACTGG-3' |
| A92E fwd | 5'-GTGCATGCAGGGCCAATTGAGCCAGGCCAGATG-3' |
| A92E rev | 5'-CATCTGGCCTGGCTCAATTGGCCCTGCATGCAC-3' |
| G94D fwd | 5'-GTGCATGCAGGGCCCATTGCACCAGACCAGATGAGAGAACC-3' |
| G94D rev | 5'-GGTTCTCTCATCTGGTCTGGTGCAATGGGCCCTGCATGCAC-3' |
| A105T fwd | 5'-GGAAGTGACATAACAGGAACTACTAGTACC-3' |
| A105T rev | 5'-GGTACTAGTAGTTCCTGCTATGTCACTTCC-3' |
| K131R fwd | 5'-CCAGTAGGAGAAATCTATAGAAGATGGATAATCCTGG-3' |
| K131R rev | 5'-CCAGGATTATCCATCTTCTATAGATTTCTCCTACTGG-3' |
| R132K fwd | 5'-GGAGAAATCTATAAAAAATGGATAATCCTGGG-3' |
| R132K rev | 5'-CCCAGGATTATCCATTTTTTATAGATTTCTCC-3' |
| K140R fwd | 5'-GGATAATCCTGGGATTAAATAGAATAGTAAGAATGTATAGC-3' |
| K140R rev | 5'-GCTATACATTCTTACTATTCTATTTAATCCCAGGATTATCC-3' |
| K158R fwd | 5'-GACATAAGACAAGGACCAAGGGAACCCTTTAGAGACTATG-3' |
| K158R rev | 5'-CATAGTCTCTAAAGGGTTCCCTTGGTCCTTGTCTTATGTC-3' |
| K170R fwd | 5'-CTATGTAGACCGATTCTATAGAACTCTAAGAGCCGAGC-3' |
| K170R rev | 5'-GCTCGGCTCTTAGAGTTCTATAGAATCGGTCTACATAG-3' |
| K182R fwd | 5'-CAAGCTTCACAAGAGGTAAGAAATTGGATGACAGAAACC-3' |
| K182R rev | 5'-GGTTTCTGTCATCCAATTTCTTACCTCTTGTGAAGCTTG-3' |
| NotI fwd | 5'-CCTTGGCTTCTTATGCGACGG-3' |
| SpeI rev | 5'-GTCCAGAATGCTGGTAGGGC-3' |
| SpeI fwd | 5'-GCTGCAGAATGGGATAGAGTGC-3' |
| ApaI rev | 5'-GTGGGAAGGCCAGATCTTCC-3' |
| D185K/D186K fwd | 5'-GACATAGTCATCTATCAATACATGAAGCTTTTGTATGTAGGATCTGACT TAG-3' |
| D185K/D186K rev | 5'-CTAAGTCAGATCCTACATACAAAAGCTTCATGTATTGATAGATGACTA TGTC-3' |
| EcoRV fwd | 5'-GTTCCCTTAGATAAAGACTTCAGG-3' |
| AgeI rev | 5'-GATATGTCCATTGGCCTTGC-3' |
| D116A fwd | 5'-CAGTAAAAACAGTACATACAGCCAATGGCAGCAATTTCACC-3' |
| D116A rev | 5'-GGTGAAATTGCTGCCATTGGCTGTATGTACTGTTTTTACTG-3' |
| AgeI fwd | 5'-CCAAAGCACTAACAGAAGTAGTACC-3' |
| EcoRI rev | 5'-CGAGTAACGCCTATTCTGCTATG-3' |
| ntTNPO3 fwd | 5'-GTTGGTTTAACTTGGGAGTTTTAGATTCGAATTTTATGGCTAACAATAA ATTACTAGCAC-3' |
| ntTNPO3 rev | 5'-GTGCTAGTAATTTATTGTTAGCCATAAAATTCGAATCTAAAACTCCCAA GTTAAACCAAC-3' |
| AflII fwd | 5'-TGTGTGCTTAAGCTCGAGTGGTGGCATGGAAGGAGCAAAGC-3' |
| KpnI rev | 5-GTAAGCTTTGAAGATGCCATG-3' |
| CMV fwd | 5'- TGTGTGTTAATTAAGCGTTGACATTGATTATTGACTAG-3' |
| CMV rev | 5'- CACACACTCGAGCCCTTGTCATCGTCGTCCTTGTAGTCCATGTTTAAA CGCTAGCCAGCTTG-3' |