Table 3.
8-week-old vs 46-week-old | RT-PCR (8-wk-old) data2 | RT-PCR (46-wk-old) data2 | |||
---|---|---|---|---|---|
NO | Gene Expression | Gene Expression | |||
C57BL/6 Male Mice | TNF-α | iNOS | TNF-α | iNOS | |
1 | Media + Macrophages (MM) | 0 | 0 | 0 | 0 |
2 | MM + LPS, 10.0 ng/mL (A) | 0.35 (100)*3 | 0.41 (100)3 | 0.38 (100)3 | 0.69 (100)3 |
3 | A + Quercetin (100 ppm) | 0.26 (74) | 0.29 (71) | 0.29 (76) | 0.47 (68) |
4 | A + δ-Tocotrienol (100 ppm) | 0.24 (69) | 0.22 (54) | 0.20 (53) | 0.28 (41) |
5 | A + Dexamethasone (10 ppm) | 0.20 (57) | 0.15 (37) | 0.27 (71) | 0.20 (29) |
*Control groups | 8-week-old vs | 46-week-old | |||
TNF-α | 0.35 (100) | 0.38 (109) | |||
iNOS | 0.41 (100) | 0.69 (168) | |||
1The assay mixture (A; contains 0.2% dimethyl sulfoxide = DMSO) was treated with LPS (10.0 ng/well) for 4 h.
2Total RNA was extracted using RNAeasy mini Kit.
3Percentage of control ratio, based on MM + LPS 10.0 ng/well.