Table 6.

Effects of LPS, LPS + dexamethasone, and dexamethasone on levels of gene expression, and biological functions in macrophages of 8-week-old C57BL/6 male mice¹.

			Regulation		Regulation			Regulation
#	Functions	LPS	Up	Down	LPS + Dexa	Up	Down	Dexa	Up	Down
1	Immune response	182	130	52	126	77	49	97	53	44
2	Cell to cell signaling	124	86	38	119	64	55	70	24	46
3	Cellular growth & proliferation	74	54	20	84	38	46	18	2	16
4	Inflammatory diseases	74	53	21	83	58	25	17	11	6
5	Respiratory diseases	44	36	8	28	24	4	17	11	6
6	Immunological diseases	39	33	6	16	12	4
7	Cancer	34	25	9	45	34	11
8	Hepatic diseases	26	23	3	35	31	4
9	Cardiovascular system	18	14	4	20	13	7	14	10	4
10	Carbohydrate Metabolism	8	5	3
11	Lipid metabolism	7	7		55	36	19	27	25	2
12	Neurological Diseases	6	5	1	26	23	3
13	Free-radical scavenging	5	4	1				5	2	3
14	Gene expression	4	4		25	21	4	18	16	2
Total		645	479	166	662	431	231	283	154	129

LPS = lipopolysaccharide; Dexa = Dexamethasone.

¹The data in this Table represents the expression of a number of genes modulated by various treatments; The macrophages were treated with LPS alone (10 ng/well), LPS (10 ng/well) + dexamethasone (10 μM), or dexamethasone (10 μM) alone as described in the experimental section. Total RNA was extracted from the treated cells and their gene expression profiles compared with the control (LPS), and untreated cells. The values here been corrected for differences in the arrays. The gene expression values are reported as average normalization log ratios. A data containing gene identifiers and their corresponding expression values were uploaded as an Excel spreadsheet using the template provided in the Ingenuity Pathways Analysis program. Similar conditions were used for data provided in Tables 8, 9, and 10.