Table 1.
1Primers | 5'-3'sequences | Product size with Unrev1025 |
---|---|---|
Human741F | GGC TTA CTT CTC TTC ATT CTC TCC T | 334 |
Cow121F | CAT CGG CAC AAA TTT AGT CG | 561 |
Pig573F | CCT CGC AGC CGT ACA TCT C | 453 |
Goat894F | CCT AAT CTT AGT ACT TGT ACC CTT CCT C | 132 |
Dog368F | GGA ATT GTA CTA TTA TTC GCA ACC AT | 680 |
Unrev1025 | GGT TGT CCT CCA ATT CAT GTT A | - |
1Primers of this multiplex PCR assay were designed based on the sequence differences in cytochrome b sequences.