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. 2012 Jan 27;7(1):e30075. doi: 10.1371/journal.pone.0030075

Figure 5. Effects of ALK1 and BMPR II siRNA on BMP-9 induced ET-1 release.

Figure 5

HPAECs were transfected with ALK1, BMPR II specific or non-targeting siRNA. Cells were starved for 16 hrs and stimulated with BMP-9 (1 ng/ml) for 24 hrs. Knock-down efficiency was determined by western blotting. The arrows denote ALK1 (62 kDa) and BMPR II (doublet 190 kDa and 145 kDa) (A). Supernatants were collected at 24 hrs post-stimulation for determination of ET-1 release by ELISA (B&C). RNA was extracted from HPAECs treated with BMPR II specific siRNA and gene expression was determined by qRT-PCR (D). Data are presented as Mean ± SEM, n = 4. #p>0.05; **p<0.01.