Figure 4. Survivin vaccine induces antigen-specific CD8 cells and entinostat enhances IFN-γ induction.

A. Tetramer analysis of splenocytes obtained from mice immunized with SurVaxM. Splenocytes were stained with anti CD8 antibodies and survivin-specific tetramers for flow cytometric analysis. Results are based upon gating of CD8+ T cells and indicate the percent of double labeled cells (CD8+/Tetramer+) with respect to specific tetramer. B, Combination treatment led to CD8⁺ IFN-γ⁺ cells induction. Mice were treated as indicated. Splenocytes were stimulated and intracellular IFN-γ staining was performed as described in Figure 1D. p<0.01 for combination vs. vehicle.