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View full-text article in PMC

. 2012 Jan 27;7(1):e31093. doi: 10.1371/journal.pone.0031093

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Sun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) The invasion potential of several human HNSCC cell lines including SCC1 (derived from the oral cavity), SCC5 (derived from the larynx), FaDu (derived from the pharynx) and OSC19 (derived from a lymph-node metastases from the tongue) were assessed using a Boyden chamber cell invasion assay. Equal numbers of cells were loaded and after 48 h incubation, invasive cells were detected on the membrane by staining with crystal violet. (B) The invasive cells were counted and the results expressed as the mean number of invasive cells±SD/microscopic field, magnification: ×10. Significantly higher versus other cells, ^* P<0.001.