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. 2012 Jan 27;7(1):e31059. doi: 10.1371/journal.pone.0031059

Figure 5. The IPCase activity was required for acid resistance in stationary phase promastigotes.

Figure 5

Promastigotes were cultured under either neutral pH (pH 7.4, A) or acidic pH (pH 5.0, B). Cell viability in stationary phase (S1–S5) was determined by flow cytometry after staining with propidium iodide. Each group of bars represents cells in the following order (from left to right): WT, iscl−, iscl−/+HA-ISCL, iscl−/+HA-BcSMase, iscl−/+5′HASPb-BcSMase-HA, and iscl−/+CnISC1. Error bars represent standard deviations from 3 independent experiments.