FIGURE 8.

Het and Hom T4826I-RYR1 channels show distinct gating behaviors. Representative channel current amplitude histograms were obtained for WT-RYR1, Het, and Hom T4826I-RYR1 under identical conditions described in the legend to Fig. 7. WT-RYR1 and T4826I-RYR1 channels show uniform current amplitudes that strongly favor the closed and full open channel states, respectively. Het T4826I-RYR1 channels exhibited broader intermediate states consistent with random association of WT-T4826I RYR1 monomers to form chimeric tetrameric channels. The insets show 1.5 s of representative current trace for each genotype.