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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Gastroenterology. 2011 Oct 29;142(2):305–315. doi: 10.1053/j.gastro.2011.10.025

Figure 4.

Figure 4

Upregulation of MMPs in day P4 Cldn7−/− intestines. (A) The mRNA levels of MMPs in Cldn7−/− intestines were greatly increased by qRT-PCR analysis. Each data point was obtained from five independent experiments. (B) MMP-3 and MMP-7 expressions, but not MMP-8, were significantly enhanced in Cldn7−/− intestines by immunoblotting examinations. The band intensity measurements (present as KO/WT) were shown on the right. (C) Increased enzymatic activities of MMPs in Cldn7−/− intestines were revealed by casein zymography gel. (D) Upregulation of MMP-3 in T84 epithelial cells with claudin-7 knockdown (KD). The insert showed the claudin-7 KD by 50 μM siRNA against claudin-7 for 72 h. The scramble siRNA was used in control cells (Con). Only MMP-3 mRNA was significantly increased with claudin-7 KD by qRT-PCR analysis. The data represented the mean values of five independent experiments and were normalized with endogenous GAPDH values. The dotted line represents no change when the ratio equals 1. (E) Real-time qRT-PCR data also showed a significant increase of MMP-3 mRNA (left) and protein (right) in human lung HCC827 cells with claudin-7 KD by specific siRNA. (F) Culture media were collected from T84 cells treated with scramble siRNA (Con) or specific siRNA against claudin-7 (siRNA). Immunoblotting displayed the increased level of MMP-3, but not MMP-7 and MMP-8 (left). MMP-7 expression was greatly increased in both control and claudin-7 KD cells after the addition of cytokine TNFα (20 ng/mL) to the culture medium for 24 h (right).