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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Gastroenterology. 2011 Oct 29;142(2):305–315. doi: 10.1053/j.gastro.2011.10.025

Figure 5.

Figure 5

Disruption of cell-cell adhesion in Cldn7−/− intestines. (A) Light micrographs of H&E stained day P5 Cldn7+/+ (+/+) and Cldn7−/− (−/−) small intestines. The arrowhead in (−/−) pointed to the sloughing epithelial cells. Magnification: ×400. (B) Electron micrographs of day P5 Cldn7+/+ (+/+) and Cldn7−/− (−/−) small intestines. The arrowhead in (−/−) pointed to the intercellular gap along the Cldn7−/− lateral membrane compared to that of Cldn7+/+ (+/+, arrowhead). The arrow in (−/−) revealed the loosening of cell-matrix connection in Cldn7−/− versus the close contact between cell and matrix in Cldn7+/+ intestines (+/+, arrow). (C) The arrows in (+/+) and (−/−) pointed to the apical TJ. The arrowheads indicated the desmosome. Magnifications: (B), ×5,000; (C), ×50,000. (D) Frozen sections of Cldn7+/+ (+/+) and Cldn7−/− (−/−) small intestines were immunostained with an apical protein marker, Ezrin. Bar: 40 μm.