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The R1500P and L1706P mutations alter the formation of myosin ordered structures in COS-7 cells. COS-7 cells were transfected with GFP-tagged myosin rod constructs as indicated. 12 h later, cells were imaged by confocal microscopy. While micrographs of cells transfected with GFP-tagged constructs alone (WT, R1500P, R1500W, L1706) are shown in Panel A, co-transfections with mCherry-tagged WT myosin rod are shown in Panel B. Bar, 10 μm.
