Figure 5. Disruption of MeCP2 methyl-DNA binding leads to deregulation of gene expression.
(a) Bdnf, Crh and Sgk mRNA expression in the hypothalamus of Mecp2T158A/y mice compared to Mecp2+/y littermates (n = 4). Bars represent mean ± SEM. * p-value < 0.05; two-tailed t-test with Bonferroni correction. (b) Bdnf, Crh and Sgk mRNA expression in the striatum of Mecp2T158A/y mice and Mecp2+/y littermates (n = 4). Bars represent mean ± SEM. * p-value < 0.05; two-tailed t-test with Bonferroni correction. (c) MeCP2 T158A mutation does not impair the association of MeCP2 with HDAC1 or Sin3a. MeCP2 immunoprecipitation from brain nuclear extracts prepared from Mecp2T158A/y and Mecp2+/y littermates are probed with indicated antibodies.