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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Neurosci. 2011 Dec 18;15(2):191–193. doi: 10.1038/nn.2994

Figure 1.

Figure 1

Rhes binds and activates mTOR. Endogenous mTOR, Raptor or Rictor and Gβl binding with overexpressed GST, GST–Rhes (WT or C263S) in striatal cells (a). Endogenous mTOR or Raptor binding with recombinant GST or GST–Rhes WT in striatal tissue (b). Purified Rhes binds directly to purified mTOR–CD (catalytic domain) (c). Rhes increases phosphorylation of S6K (Thr389), S6 (Ser240/244), 4EBP1 (Ser65) and Akt (Thr473) in serum containing and deprived (2h) striatal cells (d). Rapamycin blocks Rhes-induced phosphorylation of S6K (Thr389) but not Akt (Thr473) in HEK293 cells (e). Rhes activates mTOR directly in vitro (f). All data are expressed as means ± s.d. * p < 0.05, **p< 0.01, *** p < 0.001 compared to myc controls. Full–length blots are presented in Supplementary Figures 4–9.