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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2011 Dec 16;52(3):705–715. doi: 10.1016/j.freeradbiomed.2011.11.027

Figure 5. Induction or suppression of NQO1 altered macrophage ability to elaborate 8-isprostane after oxidant stress.

Figure 5

Alveolar macrophages were obtained from BAL in C57BL/6 and NQO1 deficient mice. The macrophages were cultured and then stimulated with either saline, LPS or PMA (A). The supernatants were collected and evaluated for 8-isoprostane and protein carbonyl concentrations. Mouse alveolar macrophage cell line (MHS) were cultured and then stimulated with either vehicle, LPS or PMA and the supernatants analyzed for 8-isoprostanes. B-D, The MHS cells were pre-treated with the NQO1 inhibitors MAC220 (B) or Dicumarol (C) followed by exposure to LPS or PMA. D, The MHS cells were pre-treated with a NQO1 inducer (β-Lapachone) prior to LPS or PMA exposure. All values represent the mean ± SEM (*P <0.05).