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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Free Radic Biol Med. 2011 Dec 1;52(4):749–756. doi: 10.1016/j.freeradbiomed.2011.11.015

Figure 3.

Figure 3

Formation of 8-oxoG* requires O2. (A) Formation of 8-oxoG* under ambient (■), O2 (▲) and N2 (◇) atmosphere. Aliquots of 8-oxoG solution were subjected to AR assays at days 1, 2, 3 and 7. (B) Changes in 8-oxoG levels as a function of time and (C) reconstitution of 8-oxoG base level by Fe2+ as determined by LC/MS. (D) AR reactivity of 8-oxoG* and H2O2 is diminished in the presence of ferrous iron. (E) Conversion of 8-oxoG to 8-oxoG* at physiological (■) and alkaline pH (◆). (F) Formation of 8-oxoG* from 8-oxoG when dissolved and stored in PBS. Results are means ±SEM (n=3–6).