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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Free Radic Biol Med. 2011 Dec 1;52(4):749–756. doi: 10.1016/j.freeradbiomed.2011.11.015

Figure 4.

Figure 4

Exposure of cells to 8-oxoG* increases cellular ROS levels. (A) MRC-5 cells were exposed to 8-oxoG* (◆), 8-oxoG (■) or H2O2 (□) and cellular ROS levels determined at times indicated. (B) Dose- and (C) cell type-dependent changes in ROS level upon 8-oxoG* exposure. (D) 8-OxoG*, but not 8-oxoG, causes changes in cellular ROS levels as shown by microscopic imaging (NIKON Eclipse Ti system). Magnification: 60x. In panels A, B, and C, cells were H2DCF-DA-loaded, and DCF fluorescence was determined at 485/528 nm. Results are means ±SEM (n=3–11).