Figure 4.

Panel A: RP-HPLC peptide map of huDKK1 peptides generated by CNBr cleavage followed by thermolytic digestion. Disulfide-containing peptides are indicated. Underlined fractions were combined and further treated with endoproteinase Lys-C and chymotrypsin. Panel B: RP-HPLC separation of peptides generated from combined endoproteinase Lys-C and chymotrypsin digestion of CBTh30 fraction as seen in panel A. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]