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. 2011 Aug 9;20(11):1814–1823. doi: 10.1002/pro.713

Figure 4.

Figure 4

Studies of the oligomerization of wild-type and mutant Ibβ TMpep peptides by SDS-PAGE and thiol-disulfide exchange assays. (A) Sequences of Ibβ TMpep and mutant peptides. The TM domain in each construct is boxed. A GGG sequence was added between the thrombin cleavage site and the Ibβ sequence to facilitate thrombin digestion. (B) SDS-PAGE of Ibβ TMpep peptide and IbβA128L TMpep, IbβG136L TMpep, and IbβH1139L TMpep mutants. (C) The thiol-disulfide exchange reaction of Ibβ TMpep peptide and the IbβA128L TMpep, IbβG136L TMpep, and IbβH1139L TMpep mutants.