Figure 5.

Effects of denaturant on EPR spectra of spin-labeled Tar. Normalized spectra for Tar in native membranes with spin labels at the indicated positions are shown in the absence (black) and presence (red) of 5M urea. Spectra were corrected for spin labels on other membrane proteins by subtracting the spectrum of native membrane vesicles containing Tar devoid of cysteine and treated with the spin-label reagent in the same way as vesicles containing the respective cysteine-containing form of Tar, scaled to total protein.