Table II.
Effective Number of Substitutions (k*) for Amino Acid Positions Based on the Results of DNA Sequencing of Functional Clones (Fig. 4)
| AA pos | Chrom | Plasmid | Alignment | AA pos | Chrom | Plasmid | Alignment |
|---|---|---|---|---|---|---|---|
| Val18 | 8.1 | 6.3 | 4.4 | Val127 | 3.2 | 7.5 | 3.0 |
| Ala20 | 3.8 | 5.2 | 2.5 | Leu130 | 4.4 | 4.3 | 1.9 |
| Asp22 | 5.6 | 8.9 | 1.0 | Thr131 | 1.5 | 1.8 | 4.2 |
| Lys44 | 1.0 | 1.8 | 1.0 | Val167 | 2.1 | 3.4 | 4.8 |
| Lys47 | 5.7 | 9.6 | 7.5 | Cys168 | 3.2 | 4.9 | 6.3 |
| Glu48 | 3.9 | 5.7 | 5.9 | Val187 | 4.2 | 4.8 | 5.0 |
| Asp71 | 1.0 | 2.5 | 1.0 | Pro189 | 3.9 | 4.9 | 1.1 |
| Lys73 | 1.0 | 1.4 | 1.0 | Gly190 | 2.3 | 2.6 | 2.5 |
| Asp76 | 1.0 | 3.6 | 1.0 | Gln201 | 1.9 | 4.4 | 1.4 |
| Thr80 | 2.6 | 4.8 | 1.1 | Val219 | 1.4 | 5.7 | 3.7 |
| His83 | 6.5 | 5.5 | 8.0 | Arg222 | 1.0 | 5.1 | 1.0 |
| Gly103 | 4.0 | 8.2 | 1.3 | Pro223 | 6.0 | 5.7 | 3.7 |