Table I.
Sequence Features and Experimental Characterization of the Designed Permuted Variants
| Design | Loop Length | Sequence | Secondary Structure | Oligomeric State | Tm (°C) |
|---|---|---|---|---|---|
| Nonpermuted | — | — | — | Dimer | 56 |
| 003 | 6 | EAGGAM | HLLLLL | Dimer | 51 |
| 004 | 5 | EAGGM | HLLLL | Dimer | 49 |
| 005 | 6 | EAAGGM | HLLLLL | Multimer | NT |
| 006 | 5 | EAAGM | HLLLL | Dimer | 48 |
| 007 | 5 | EGGGM | HLLLL | Dimer | NT |
| 008 | 7 | EAGGAAM | HLLLLLL | Insoluble | - |
The solution oligomeric state was characterized by SEC/SLS. The melting temperatures were determined by circular dichroism spectroscopy.