FIG. 1.

Microfluidic gradient generator device. (A) Photograph of device filled with dye for visualization of gradient. Microcapillaries (not visible in the image) connect the source and sink channels to the cell culture chamber, enabling formation of a stable gradient across the cell culture chamber. B-C. Quantification of equilibrium gradients of Dextran/Texas Red (B) and VEGF-165/Texas Red (C) in the high-density biomaterial (5 μg/mL fibronectin and 2.7 mg/mL collagen). For each condition, three individual fluorescence intensity measurements (thin gray lines) and a least-squares linear regression (thick black lines) are shown. VEGF, vascular endothelial growth factor. Color images available online at www.liebertonline.com/tea