FIG. 1.

Representative properties of HUVEC spheroids. Formation of HUVEC spheroids in vitro (a–c) and alteration in gene expression by spheroid formation in vitro (d) at 3 days of culture. (a) H&E staining. Scale bar=100 μm. (b) Scanning electron microscopy image. Scale bar=100 μm. (c) TUNEL staining. Scale bar=100 μm. (d) RT-PCR and (e) western blot analysis. HUVEC, human umbilical vein endothelial cell; H&E, hematoxylin and eosin; RT-PCR, reverse transcription–polymerase chain reaction. Color images available online at www.liebertonline.com/tea