Skip to main content
. 2011 Oct 17;18(3-4):310–319. doi: 10.1089/ten.tea.2011.0193

FIG. 5.

FIG. 5.

FIG. 5.

Enhanced angiogenesis by transplantation of HUVECs as spheroids. Double-immunofluorescent staining for HNA and mouse-specific (a) vWF and (c) SM α-actin of the ischemic hindlimb tissues retrieved at 28 days after HUVEC transplantation. Scale bars indicate 200 μm at 100×and 100 μm at 200×. The quantification of (b) capillary density and (d) arteriole density in the ischemic region (*p<0.05 between groups compared). Quantification of the number of HUVECs incorporated into (e) capillaries and (f) arterioles in the ischemic region (*p<0.05). (g) RT-PCR analysis of human-specific vWF, SM α-actin, HNA, and proliferating cell nucleus antigen of the ischemic hindlimb tissues at 28 days after transplantation. (h) Western blot analysis for ICAM, VCAM, NG2, and MMP-2 in the ischemic hindlimb tissues at 28 days after transplantation. White arrow indicates double stained cells (vWF+/HNA+, or SM α-actin+/HNA+). vWF, von Willebrand factor; SM, smooth muscle; ICAM, intercellular adhesion molecule; VCAM, vascular cell adhesion molecule; MMP, matrix metalloproteinase. Color images available online at www.liebertonline.com/tea