Table 3.
Substrate | Parameter | PcT | BT | PcT/BT |
---|---|---|---|---|
BAPA | K m (mM) | 0.037 | 0.57 | 0.063 |
K cat (sec−1) | 3.7 | 1.7 | 2.2 | |
K cat/K m (mM−1s−1) | 100 | 2.9 | 35 | |
| ||||
TAME | K m (mM) | 0.0015 | 0.021 | 0.074 |
K cat (sec−1) | 108 | 86 | 1.3 | |
K cat/K m (mM−1s−1) | 71,206 | 4,160 | 17 | |
| ||||
N-Boc-L-Ala-OpAm (4a) | K m (mM) | 0.0015 | 0.021 | 0.071 |
K cat (sec−1) | 2.0 | 1.2 | 1.6 | |
K cat/K m (mM−1s−1) | 1,449 | 619 | 2.3 |
PcT, Pacific cod trypsin; BT, Bovine trypsin.