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. 2011 Sep 19;2011:912382. doi: 10.4061/2011/912382

Table 3.

Kinetic parameters for Pacific cod trypsin-catalyzed hydrolysis.

Substrate Parameter PcT BT PcT/BT
BAPA K m (mM) 0.037 0.57 0.063
K cat (sec−1) 3.7 1.7 2.2
K cat/K m (mM−1s−1) 100 2.9 35

TAME K m (mM) 0.0015 0.021 0.074
K cat (sec−1) 108 86 1.3
K cat/K m (mM−1s−1) 71,206 4,160 17

N-Boc-L-Ala-OpAm (4a) K m (mM) 0.0015 0.021 0.071
K cat (sec−1) 2.0 1.2 1.6
K cat/K m (mM−1s−1) 1,449 619 2.3

PcT, Pacific cod trypsin; BT, Bovine trypsin.