Table 4.

Yield of Pacific cod trypsin-catalyzed peptide synthesis.

Entry no.	Acyl donor (no.)	Reaction time (hr)	Product (No.)	Yield (%)
1	N-Boc-L-Ala-OmAm (1a)	48	N-Boc-L-Ala-L-Ala-pNA (13a)	44
2	N-Boc-L-Ala-OmGu (2a)	24	N-Boc-L-Ala-L-Ala-pNA (13a)	37
3	N-Boc-L-Ala-OmAM (3a)	24	N-Boc-L-Ala-L-Ala-pNA (13a)	55
4	N-Boc-L-Ala-OmGM (4a)	12	N-Boc-L-Ala-L-Ala-pNA (13a)	51
5	N-Boc-L-Ala-OpAm (5a)	0.2	N-Boc-L-Ala-L-Ala-pNA (13a)	58
6	N-Boc-L-Ala-OpGu (6a)	0.2	N-Boc-L-Ala-L-Ala-pNA (13a)	60
7	N-Boc-L-Ala-OpAM (7a)	5	N-Boc-L-Ala-L-Ala-pNA (13a)	59
8	N-Boc-L-Ala-OpGM (8a)	5	N-Boc-L-Ala-L-Ala-pNA (13a)	59
9	N-Boc-L-Ala-O(1-4)GN (9a)	5	N-Boc-L-Ala-L-Ala-pNA (13a)	56
10	N-Boc-L-Ala-O(1-5)GN (10a)	5	N-Boc-L-Ala-L-Ala-pNA (13a)	55
11	N-Boc-L-Ala-O(1-6)GN (11a)	24	N-Boc-L-Ala-L-Ala-pNA (13a)	52
12	N-Boc-L-Ala-O(2-5)GN (12a)	50	N-Boc-L-Ala-L-Ala-pNA (13a)	59
13	N-Boc-D-Ala-OmAm (1b)	74	N-Boc-D-Ala-L-Ala-pNA (13b)	12
14	N-Boc-D-Ala-OmGm (2b)	74	N-Boc-D-Ala-L-Ala-pNA (13b)	52
15	N α-Boc-D-Ala-OmAM (3b)	74	N-Boc-D-Ala-L-Ala-pNA (13b)	38
16	N-Boc-D-Ala-OmGM (4b)	74	N-Boc-D-Ala-L-Ala-pNA (13b)	58
17	N-Boc-D-Ala-OpAm (5b)	0.2	N-Boc-D-Ala-L-Ala-pNA (13b)	70
18	N-Boc-D-Ala-OpGu (6b)	0.2	N-Boc-D-Ala-L-Ala-pNA (13b)	75
19	N-Boc-D-Ala-OpAM (7b)	8	N-Boc-D-Ala-L-Ala-pNA (13b)	76
20	N-Boc-D-Ala-OpGM (8b)	48	N-Boc-D-Ala-L-Ala-pNA (13b)	74
21	N-Boc-D-Ala-O(1-4)GN (9b)	5	N-Boc-D-Ala-L-Ala-pNA (13b)	57
22	N-Boc-D-Ala-O(1-5)GN (10b)	97	N-Boc-D-Ala-L-Ala-pNA (13b)	61
23	N-Boc-D-Ala-O(1-6)GN (11b)	97	N-Boc-D-Ala-L-Ala-pNA (13b)	33
24	N-Boc-D-Ala-O(2-5)GN (12b)	74	N-Boc-D-Ala-L-Ala-pNA (13b)	23

1 mM acyl donor, 20 mM acyl acceptor, 10 μM PcT, respectively, in MOPS (50 mM, pH 8.0, containing 20 mM CaCl₂) : DMSO = 1 : 1, at 25°C.