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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 May 7;32(23):5478–5488. doi: 10.1016/j.biomaterials.2011.04.026

Figure 1.

Figure 1

(A) A process flow diagram for micorpatterning hydrogel microwells on glass. In addition to acrylate moieties used to couple hydrogel microstructures to glass, silane layer also contains thiol groups for covalent linking of proteins. (B) Strategy for immobilizing biomolecules inside microwells. Mixed silane layer was activated using a hetero-bifunctional cross-linker, and then incubated with avidin and biotin-antibody. Throughout this paper the sensitivity of immunoassays constructed on mixed silanes was compared to immunoassays created by physical adsorption of avidin on mono-functional acrylated silanes followed by immobilization of biotin-antibody.