Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 May 7;32(23):5478–5488. doi: 10.1016/j.biomaterials.2011.04.026

Figure 2.

Figure 2

Figure 2

ToF-SIMS analysis of silane modification and avidin immobilization on glass substrates. (A) Schematic representation of surface bombardment with C60 projectiles. Impact of each projectile creates a crater 5 to 10 nm in diameter and sends ions from this volume into the SIMS instrument. Ions emitted from each impact are accounted for and provide important information about spatial co-existence of chemical species. (B) Comparison of negative ion mass spectra of glass substrates modified with acrylated silane (up) vs. a bifunctional thiol/acrylated silane (bottom). This analysis reveals the presence of both acryl and thiol functional groups in the mixed silane assembled on glass. (C–D) The negative ion mass spectra collected from glass substrates after attachment of linker (up) and streptavidin (bottom). These spectra demonstrate presence of masses assigned to peptide bonds as well as to amino acids present in streptavidin.