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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 May 7;32(23):5478–5488. doi: 10.1016/j.biomaterials.2011.04.026

Figure 4.

Figure 4

Fluorescence microscopy analysis of streptavidin adsorption in microwells. Individual microwells were 100 µm in diameter. (A) Physical adsorption of streptavidin-Alexa546 in microwells containing acrylate groups in the attachment sites. Note very weak fluorescence signal observed in the microwells that is comparable to non-specific fluorescence of the hydrogel walls. (B) Streptavidin-Alexa546 covalent immobilized in microwells modified with thiol/acrylate silane layer. (C) Quantitation of fluorescence intensity showing ~6 times higher fluorescence in microwells created on a mixed silane layer.