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Schematic describing cytokine release studies. Step 1: Macrophages were captured inside microwells pre-coated with anti-TNF-α Abs. Cells were mitogenically activated to induce cytokine production. Step 2: Binding of secreted TNF-α inside the microwells was determined by sandwich immunoassay. Fluorescence cytokine signal was co-localized with specific cytokine-producing macrophages.
