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Exogenously applied PGE2 contracts the murine urinary bladder strips in a concentration-dependent manner (A; np = 11, na = 6) with an EC50 of 9.2 µM. (B) A sample trace of the effects of PGE2 (50 µM) on such a muscle strip (np = 20, na = 10). The increase in tone is followed by phasic activity. PGE2 (50 µM) increased tone (C; np = 20, na = 10) and the frequency of phasic contractions (D; np = 14, na = 7). **P < 0.01 and ***P < 0.001, compared to control.
