Figure 7.

DF 2156A inhibited PMN infiltration and tissue damage in liver I/R. Reperfusion injury was induced by 1 h ischaemia of the liver followed by 3, 12 or 24 h reperfusion. Ischaemic rats were treated i.v. with DF 2156A (15 mg·kg⁻¹; solid columns) or vehicle (open columns) 15 min before reperfusion (six animals per experimental group). (A) PMNs were identified by naphthol AS-D chloroacetate technique for non-specific esterase. Red-stained PMNs were counted in 20 non-consecutive, randomly chosen 400× histological HPF. I/R injury was evaluated by plasma activity of ALT (B) and histopathological analysis of post-ischaemic liver in vehicle (C) or DF 2156A-treated (D) groups. Arrows indicate necrotic hepatocytes. Data are mean ± SEM from one experiment representative of three performed. *P < 0.05; **P < 0.01 versus respective vehicle-treated group by Student's t-test.