Figure 3.

rVP1 suppresses invasion and MMP-2 activity of SKOV3 cells. (A) SKOV3 cells were pretreated with control IgG or anti-integrin β1 antibodies (2 µg·mL⁻¹) for 30 min followed by rVP1 treatment for 24 h in serum-free medium. The invasive ability of the cells was determined by Matrigel invasion assay. Data represent means ± SD of three independent experiments. (B) Cells were pretreated with control IgG or anti-integrin β1 antibodies (2 µg·mL⁻¹) for 30 min followed by 0.3 µM rVP1 treatment for 24 h. The MMP-2 enzyme activity in the cell-cultured medium was analysed by a gelatinolytic zymography assay. (C, D) SKOV3 cells were transfected with control vector, plasmid encoding wild-type Akt (Akt-WT), constitutively active Akt (Akt-DA) or dominant negative Akt (Akt-DN) for 36 h, followed by rVP1 treatment for 24 h in serum-free medium and the invasion and MMP-2 activity of the cells was determined. Also shown are expression levels of phosphorylated Akt or total Akt, as determined by Western blotting. Data represent means ± SD of three independent experiments *P < 0.01. NS, not significant.