Figure 10.

NOS activity in BAEC in response to ONOO^- (SIN-1) and H₂O₂. NOS activity was determined in cells after exposure to SIN-1 (SIN, 25 µM, 24 h) or H₂O₂ (HP, 25 µM, 8 h) as production of [³H]-l-citrulline from [³H]-l-arginine (pmol mg⁻¹ protein 40 min⁻¹). NOS function also was determined in BAEC transfected with siRNA to p115-RhoGEF (p115) or control scrambled (SC) siRNA before treatment with oxidants. Values are represented as % of control. Data represent mean ± SEM from four independent experiments carried out in triplicates. Significant change from control treatment is represented as *P < 0.05.