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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Integr Biol (Camb). 2011 May 10;3(6):696–705. doi: 10.1039/c0ib00137f

Figure 4.

Figure 4

Confocal images of B16.F10 (a, b) and EMT6 (c, d) cells stained with CellTracker Green CMFDA for visualization of cell viability following 12-hr co-incubation with mCherry-expressing E. coli (red). E. coli cells were either co-expressing invasin (inv+) (a, c) or expressing mCherry alone (inv−) (b, d). To clearly demonstrate inv− bacterial proliferation-induced tumor cell death, red and green fluorescence channels in images b and d were split into two images to show distributions of live tumor and bacterial cells separately. They are denoted as b1 (green channel of b), b2 (red channel of b), d1 (green channel of d), and d2 (red channel of d).