Fig. 2.

Phenotypic characterisation of ‘ex vivo’ MSCs in ICBM and LBFBM aspirates. A — Gating strategy: MNCs were gated on FSC/SSC to exclude cell debris and DAPI exclusion was used to remove non-specific fluorescence by dying/dead cells. The CD45^−/low CD271⁺ cells were then gated as the population of interest and expression of other markers (CD73 and CD31 shown as examples) was characterised within this population. B — The expression of classic MSC markers CD73, CD90 and CD105 and the lack of CD34, CD31, CD61, CD19 and CD33 on gated CD45^−/lowCD271⁺ cells from ICBMA (white) and LBFBM (grey)(n = 3 matched donors, error bars represent SDs). C — Histograms showing the expression of markers on gated CD45^−/lowCD271⁺ cells from ICBMA (white) and LBFBM (grey) from one representative donor. Dotted lines show isotype control.