Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan 9;109(4):1317–1322. doi: 10.1073/pnas.1016199109

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Fig. 1. — BTC stimulates neurosphere growth. (A) Adult mouse NSCs were grown in Transwell devices in the presence of ECs and were treated with 10 μg/mL anti-BTC blocking antibody or an isotype control. Neurosphere number is expressed as the average per well ± SE. (B and C) NSCs were stimulated as indicated with 20 ng/mL FGF, EGF, and/or BTC. Neurosphere number (B) and diameter (C) were determined after 10 d and expressed as the average ± SE. *P < 0.05; **P < 0.005; and ***P < 0.0005 vs. FGF or as indicated (one-way ANOVA plus Bonferroni posttest). (D) Primary neurospheres were generated in the presence of BTC (20 ng/mL) for 10 d, after which they were disgregated, seeded at the initial concentration, and grown with BTC to generate secondary neurospheres. 1^ry, primary; 2^ry, secondary. (E) NSCs were grown as in B in the presence of FGF (20 ng/mL) plus the indicated concentrations of EGF or BTC, and the diameter ± SE was quantified.