Fig. 5.

Effect of PI3Kγ deficiency on disease progression and pathogenesis of cutaneous L. mexicana infection. C57BL/6 mice genetically deficient of the p110γ subunit of PI3Kγ (PI3Kγ^−/− mice) were infected by inoculating 10⁴ metacyclic L. mexicana promastigotes into the left ear dermis. (A) Disease progression was monitored by measuring the thickness of infected ears using a dial-gauge micrometer at weekly intervals. Data are expressed as the increase in the thickness of infected ears compared with uninfected ears. Data shown are from three independent experiments (n = 15 mice per group) and are presented as mean lesion size ± SE. For lesion sizes, P < 0.05 by an unpaired Student's t test. (B) Parasite loads at weeks 3, 6, and 9 postinfection. Data are expressed as the mean log parasite dilution ± SE and are representative of three independent experiments with similar results. For determination of parasite burdens, *P < 0.05 by a Mann–Whitney U prime test.