Fig. 3.
Effect of digoxin on the expression of genes regulated by HIF-1. (A) Quantitative real-time RT-PCR analysis of GLUT1, TRPC1, and NHE1 mRNA levels in lung tissue from chronically hypoxic mice treated with saline or digoxin (1 mg/kg per day). (B) Analysis of GLUT1, TRPC1, and NHE1 mRNA levels in PASMCs exposed to hypoxia ex vivo (4% O2; 60 h). Levels of target gene mRNAs were normalized to cyclophilin mRNA levels within samples, and data are expressed as fold change relative to levels measured in normoxia under the same treatment conditions (n = 3–4 per group). *Significant difference compared to saline (in panel A) or vehicle (in panel B).