Fig. 5.

Inside-out signals are not required for the phagocytosis of B. burgdorferi. (A) RAW264.7 cells were incubated with Bb914 (m.o.i. = 25, green histogram) or MnCl₂ (1 μM, red histogram), washed, and stained with the mAb CBRM1/5. The cells were fixed and analyzed by flow cytometry. (B) RAW264.7 cells were incubated with increasing concentrations of MnCl₂ (0.5 μM, orange histogram; 1 μM, red histogram) or left unstimulated (black histogram), washed, and assessed for their phagocytic activity using Bb914 (m.o.i. = 25). (C) CHO-CR3 (Upper) and CHO-CR3/CD14 cells (Lower) were incubated with Bb914 (green histograms) or MnCl₂ (red histograms), as before. The cells were stained with the mAb CBRM1/5, fixed, and analyzed by flow cytometry.