Fig. 2.

Mitotic karyotype of a T. miscellus plant showing an additive chromosome complement. Metaphase chromosomes (from plant 2875–1-1) were first subjected to FISH (top row) using probes for 35S rDNA (green), a centromeric repeat (TPRMBO; red), and a subtelomeric repeat (TGP7; yellow). The same spread was then reprobed with total genomic DNA (GISH; middle row) of T. dubius (green) and T. pratensis (red); chromosomes were counterstained with DAPI (gray). The lower row shows the same chromosomes with only DAPI staining (blue). Each chromosome is present in two copies (disomic). Examples of chromosomes that are homologs and homeologs are indicated. (Scale bar: 5 μm.)