Fig. 3.

Mitotic karyotype of an additive T. miscellus plant probed with dispersed repetitive DNA. Metaphase chromosomes (from plant 2875-B-5) were first subjected to FISH (upper row) using a mixture of two probes for DNA repeats abundant in only one of the diploid parental genomes, T. pratensis (pra001; red) and T. dubius (dub005; green). In addition, a subtelomeric repeat (TGP7; yellow) present in both subgenomes was included, and DNA was counterstained with DAPI (gray; visible where the probe signal is less intense). The same chromosome spread was then reprobed with total genomic DNA (lower row) of T. dubius (green) and T. pratensis (red). (Scale bar: 5 μm.)