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. Author manuscript; available in PMC: 2012 Jan 30.
Published in final edited form as: J Comp Neurol. 2011 Dec 15;519(18):3802–3814. doi: 10.1002/cne.22734

Figure 2.

Figure 2

Preservation of antigenicity for PSD-95 and TPOH after multiple rounds of antibodies staining/elution in array tomography. A–C: The same 70-nm ultrathin section labeled against PSD-95 (red) and TPOH (white) in three consecutive rounds of staining/elution showing highly similar patterns of labeling. Examples of the PSD-95 immunolabeling repeated in each round are shown within yellow circles. Controls where primary antisera were omitted confirmed immunolabeling was completely removed by elution procedure. D,E: Merged images from the same 70-nm section labeled for TPOH (upper panel) and PSD-95 (lower panel) in three consecutive rounds of staining/elution. The first round of staining is pseudocolored in blue, the second in red, and the third one in green. Overlapping pixels are shown in white. F: Quantitative analysis of PSD-95 puncta density either associated or not with TPOH-positive areas for the same stack of images after three rounds of antibodies staining/elution. Scale bars = 10 μm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]