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. Author manuscript; available in PMC: 2012 Jan 30.
Published in final edited form as: J Comp Neurol. 2011 Dec 15;519(18):3802–3814. doi: 10.1002/cne.22734

Figure 6.

Figure 6

Quantitative analysis of VGLUT/PSD-95 puncta appositions in the mouse DR. A,B: Density of VGLUT/PSD-95 puncta appositions, and their association with 5-HT cells in the ventral (vDR) and dorsal (dDR) parts of the DR, respectively. C–E: Serial section images of appositions containing VGLUT1 (green), VGLUT2 (magenta), or VGLUT3 (light blue), respectively, in association with 5-HT cells (white). F: Cross-correlation analysis of pixels for PSD-95 and VGLUT1 (green), VGLUT2 (magenta), or VGLUT3 (light blue) puncta in the DR. The control analysis of the poorly colocalized VGLUT1 and VGLUT2 puncta is also shown (black). *P < 10−4 vs. either VGLUT1 or VGLUT3. **P < 0.02 vs. VGLUT3. ΔP < 0.004 for dDR VGLUT/PSD-95 vs. vDR. ***P < 0.05 vs. either VGLUT1 or VGLUT3. ΔΔP < 0.003 for dDR VGLUT1/PSD-95/5-HT vs. vDR. Scale bar = 2 μm in E (applies to C,D). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]