Skip to main content
. Author manuscript; available in PMC: 2012 Jan 30.
Published in final edited form as: Ann Biomed Eng. 2010 Oct 29;39(2):674–687. doi: 10.1007/s10439-010-0197-x

FIGURE 8.

FIGURE 8

Intracellular ROS mediates induction of DNA damage by DBD plasma. (a) Melanoma cells were treated with the indicated dose of plasma as described. After 1-h incubation, lysates were prepared and resolved by SDS-PAGE, and representative immunoblots with antibody to γ-H2AX (top) or α-tubulin (bottom) are shown. (b) Melanoma cells were incubated for 2 h with 4 mM N-acetyl-L-cysteine (NAC) (+) or cell culture medium (−), followed by treatment with the indicated dose of DBD plasma. γ-H2AX (top) or α-tubulin (bottom) was detected by immunoblot of cell lysates prepared 1-h after plasma treatment.