Figure 4. Maintaining Emc in the furrow is sufficient to prevent elevation of Da.
Flip-on clones expressing emc using act-Gal4 and emcEP3620 line are marked by GFP (green).
(A) Emc expression (magenta) from emcEP3620 elevated Emc level slightly (yellow arrow). Within the furrow (white arrow), Emc level was comparable to the normal level ahead of the furrow.
(B) Clonal expression of Emc prevented Da expression from rising in the furrow (yellow arrow). Da expression level was unaffected elsewhere.
Genotype: (A and B) ywhsF; emcEP3620/ act>CD2>Gal4, UAS-GFP.