Table 1.
Primer Sequences used for RT-PCR to assess gene expression of markers of retinal progenitors and mature retinal neurons by hMSC and non-pigmented CE cells.
| Name | Forward sequence | Reverse sequence | cDNA location (base pairs) | Product Size (base pairs) |
|---|---|---|---|---|
| CRALBP | CGTGGCGGAGAGGGTGCAAG | GGTGCAGCGGACAGCCTCTG | 360–528 | 169 |
| CHX10 | AGCTAGAGGAGCTGGAGAAG | CATGATGCCATCCTTGGCTG | 556–846 | 258 |
| GAPDH | CCACCCATGGCAAATTCCATGGCA | TCTAGACGGCAGGTCAGGTCCACC | 188–785 | 598 |
| MERTK | GGGAGATCGAGGAGTTTCTC | CGGCCTTGGCGGTAATAATC | 2008–2325 | 388 |
| MITF-A | TGAAGAGCCCAAAACCTATTACGA | GATCAATCAAGTTTCCCGAGACAG | 170–906 | 736 |
| NOTCH1 | GCTGGACTGGTGAGGACTG | AGCCCTCGTTACAGGGGTT | 977–1153 | 177 |
| NRL | GGCTCCACACCTTACAGCTC | GGCCCATCAACAGGGACTG | 118–311 | 212 |
| PAX6 | AGATGAGGCTCAAATGCGAC | GTTGGTAGACACTGGTGCTG | 1102–1385 | 302 |
| Rhodopsin | GCTTCCCCATCAACTTCCTCA | AGTATCCATGCAGAGAGGTGTAG | 152–285 | 156 |
| RPE65 | GCCCAGGAGCAGGACAAAAG | GCGCATCTGCAAGTTAAAACCA | 1529–1775 | 247 |
| SHH | TGCTGCTGCTGGCGAGATGT | AATCGCTCGGAGTTTCTGGTG | 153–369 | 217 |
| S-Opsin | TAGCAGGTCTGGTTACAGGATG | GAGACGCCAATACCAATGGTC | 347–474 | 148 |
| SOX2 | GGCAGCTACAGCATGATGC | TCGGACTTGACCACCGAAC | 932–1149 | 236 |
| ZO-1 | CCAGAATCTCGGAAAAGTGC | ACCGTGTAATGGCAGACTCC | 2736–23085 | 397 |