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. Author manuscript; available in PMC: 2013 Jan 18.
Published in final edited form as: Bioconjug Chem. 2011 Dec 23;23(1):42–46. doi: 10.1021/bc200496e

Figure 1.

Figure 1

Delivery of GFP. A. The cross linker SPDP is used to modify the amine group of a lysine residue (blue) to pyridyl-dithiol. Reduction using TCEP exposes a free thiol (yellow), which reacts with the N-terminal maleimide moiety of SPv to form a thioether. B. U87 cell treated with 20 nM GFP-SPv conjugate; nuclear stain (left), GFP fluorescence (center), merged channels (right). (Scale bar = 10 µm).