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. Author manuscript; available in PMC: 2013 Jan 18.
Published in final edited form as: Bioconjug Chem. 2011 Dec 23;23(1):42–46. doi: 10.1021/bc200496e

Fig. 2.

Fig. 2

Delivery of functionally-expressed DNA fragment. A. A DNA fragment coding for GFP was generated by PCR with the introduction of Alexa-647 fluorophore (red) and a free amine (blue). SPDP was used to conjugate the DNA fragment to SPv-NLS, which contains an N-terminal maleimde, followed by a nuclear localization sequence (orange) and the SP sequence. B. Hela cells transfected with the NK1R show internalization of the DNA conjugate (red) with both cytoplasmic and nuclear localization after 2 hrs of treatment with 200 pM DNA-SPv-NLS conjugate, and C. GFP expression (green) after 48 hrs of treatment. D. Hela cells treated with DNA fragment not conjugated to SP. (Scale bar = 10 µm).